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Open Access Highly Accessed Research article

High glucose induces apoptosis and suppresses proliferation of adult rat neural stem cells following in vitro ischemia

Jian Chen1, Yang Guo1, Wei Cheng1, Ruiqing Chen1, Tianzhu Liu2, Zhenzhou Chen2 and Sheng Tan1*

Author Affiliations

1 Key Laboratory of Brain Function Repair and Regeneration of Guangdong, Department of Neurology, Zhujiang Hospital, Southern Medical University, Guangzhou, China

2 Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou, China

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BMC Neuroscience 2013, 14:24  doi:10.1186/1471-2202-14-24

Published: 4 March 2013

Additional files

Additional file 1: Figure S1:

The viability of NSCs was examined by MST assay after 24 h, 48 h or 72 h of growth. Neural stem cells were exposed to 2 mM, 7 mM or 17.5 mM D-glucose. The absorbance at 490 nm is directly proportional to the number of cells in each well at each time point. Figure S2. The relative increase in the number of neural progenitor cells in each group is represented by the ratio of 3-day viability to 1-day viability. The relative increase in NSCs exposed to 2 mM and 7 mM glucose was less than that in NSCs exposed to 17.5 mM glucose. The data are presented as the mean ± SD (n = 6) of the relative increase in cell number. *P < 0.05; NS, not significantly different.

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