Figure 1.

The time courses of extracellular H2O2 concentration changes following impact injury to the rat spinal cord in saline-treated, MPSS-treated, and MnTBAP-treated groups. The procedure was described in detail in the Methods section. After three microdialysates were collected at 20-min intervals to establish a basal level, a weight was dropped at time 0 onto the cord (25 g·cm), and sampling was continued for another 5 h at 20-min intervals. A single dose of MnTBAP (4 mg/kg) or the equivalent intrathecal standard regimen of MPSS (bolus dose plus maintenance dose for a total of 9.24 mg/kg as described in the Methods) was administered into the intrathecal space of the cord through a catheter implanted in the terminal cistern. The samples were collected in vials containing a solution of FeCl2 and salicylate. H2O2 in the microdialysates were converted to OH in the collecting vials by the Fenton reaction. The OH produced in the collecting vials rapidly attacks salicylate to produce 2,3- and 2,5-DHBA. The salicylate hydroxylation products 2,3- and 2,5-DHBA were analyzed by HPLC with electrochemical detection. The 2,3-DHBA was calibrated to the amount of H2O2. Clearly, both MnTBAP and MP reduced H2O2 to its basal level.

Liu et al. BMC Neuroscience 2013 14:23   doi:10.1186/1471-2202-14-23
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