itpr function in neurons regulates body weight and fat storage under starvation. (A) Flies with pan-neuronal and peptidergic knock down of itpr show a significant increase in their body weights post feeding for 144 hrs as well as post starvation for 72 hrs as compared to the RNAi heterozygote control. Over-expression of itpr+ in peptidergic neurons in the mutant background rescues body weight in both fed and starved conditions as compared to the mutant. The body weights are normalized to the respective 0 hrs time point for each genotype. (B) Quantification of TAGs from fly abdomens. Pan-neuronal knock down of itpr shows elevated levels of TAGs under fed and starved conditions when compared to the dsitpr/+ control. Peptidergic knock down of itpr shows elevated TAGs post starvation as compared to the RNAi control. itpr+ over-expression using the dimm driver in the itprku mutant background restores the TAG levels near wild type under both fed and starved conditions. The TAGs are first normalized to total protein and then to the respective 0 hrs time point for each genotype. *p < 0.05; Student’s t test; difference significant as compared to the corresponding time point of the RNAi control unless otherwise indicated.
Subramanian et al. BMC Neuroscience 2013 14:157 doi:10.1186/1471-2202-14-157