Synergistic and sustained phosphorylation of Erk and JNK upon combinatorial NGF and PACAP treatment. (a) Time-course of Erk and JNK phosphorylation at 20 and 60 minutes following NGF (0-50 ng/ml)-PACAP (0-100 ng/ml) (NP) treatment. Phosphorylation levels of the proteins were analyzed by western blotting, and normalized to the levels of actin. Fold-changes in (b) pErk and (c) pJNK were quantified by densitometry. Data for 50 ng/ml NGF and 100 ng/ml PACAP (highlighted in blue) were plotted and analyzed for synergism. Significant differences between combinatorial experimental treatment of NGF-PACAP and summation of their individual effects were calculated using the paired Student’s t-test. A value of p < 0.05 was considered significant (**p < 0.01). Graphs showing synergistic Erk and JNK phosphorylation at the other ligand concentrations were plotted in Additional file 3: Figure S3a & S3b. Western blot data and quantified results for pP38 and pAkt were plotted in Additional file 2: Figure S2a-S2c.
Seow et al. BMC Neuroscience 2013 14:153 doi:10.1186/1471-2202-14-153