MnTBAP + L-NA reduced SCI-induced apoptosis. Spinal cord sections were obtained at 12 h post SCI as in Figure 7. Upper: photomicrographs of TUNEL-stained sections at 2 mm rostral from the epicenter in the gray matter of the cord. A-C: lower magnification and D-F: higher magnification of A-C. A and D: sham control; B and E: injured section; C and F: injured section treated with MnTBAP + L-NA. Treatment by MnTBAP + L-NA clearly reduced the number of TUNEL-positive cells. The TUNEL-positive cells at the sections 2 mm from the epicenter were counted in both the gray and the white matter and the counts compared between the combination-treated and vehicle-treated groups (lower panel). a: Number of TUNEL-positive cells in the gray matter. b: Number of TUNEL-positive cells in the white matter. Error bars, mean ± SEM. Asterisk indicates a significant difference between the two treatment groups (p < 0.05). The combination of MnTBAP + L-NA significantly reduced the number of TUNEL-positive cells in both the gray and the white matter.
Ling et al. BMC Neuroscience 2013 14:146 doi:10.1186/1471-2202-14-146