Membrane permeable analogues 8-Br-cAMP and 8-Br-cGMP do not increase glial cell migration in a scratch wound assay. (A-C) Incubation of canine olfactory ensheathing cells and Schwann cells with 8-Br-cAMP (D-F) or 8-Br-cGMP showed no significant increase of glial migration rate after 8 hours. (A) Ensheathing cells from the olfactory bulb showed a slight increase to 125% of control at 10 μM to 500 μM 8-Br-cAMP. However, this effect was not significant. (B-C) OM-OECs and Schwann cells showed no increase under cAMP treatment. (D-F) Ensheathing cells from the olfactory bulb showed a slight, not statistically significant increase to 120% compared to controls, at 10 μM 8-Br-cGMP whereas OM-OECs and Schwann cells showed no change in migration rate under cGMP treatment. Histograms present means ± SEM of 4 separate experiments (all cell types for 8-Br-cAMP) and 4 (OB-OECs, OM-OECs) and 3 (Schwann cells) for 8-Br-cGMP. Statistical analysis was performed using an ANOVA and the Newman-Keuls post-hoc test.
Roloff et al. BMC Neuroscience 2013 14:141 doi:10.1186/1471-2202-14-141