Additional file 1.
Protein profile of CE photoreceptors’ differentiation. CE neurospheres were cultured in the presence of PN1CM for 20 days. Immunostaining indicated a small subset of cells with GFP fluorescence (Nrl-GFP, green) demonstrating the activation of Nrl promoter, immunoreactive to GFP antibody (GFP-Ab, red), indicating the specificity of the Nrl-GFP-fluorescence (A,B). Co-localization of Nrl-GFP fluorescence (green) and rhodopsin immunoreactivities (red), detected by RetP1 (C) and Rho4D2 (D). Detailed immunostaining on Retp1 positive cells indicating membrane localization of rhodopsin (E) in differentiated rat CE cells.
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Del Debbio et al. BMC Neuroscience 2013 14:130 doi:10.1186/1471-2202-14-130