Additional file 5: Figure S4.

Alignment of FM1-43 with IR puncta after post-hoc ICC. A) Immunocytochemical images of FM1-43 in blue (pseudo colour), Alexa 488 (green) and Alexa 594 (red). Upper panels show ROIset superimposition and lower panels show line plot along a fiber. Note that the different ROIs are well fitted to the puncta in the different channels, this step is useful to visually check the alignment of the three channels. B) Arbitrary fluorescence units plot over the three channels of the ROIs indicated in A).

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Ramírez-Franco et al. BMC Neuroscience 2013 14:127   doi:10.1186/1471-2202-14-127