Graphical abstract of FM1-43 experiments and subsequent post-hoc immunolabelling. Each number represents a different step in the protocol detailed throughout the paper. Briefly, 7DIV cultured cerebellar granule cells (1) were used to assess synaptic efficiency by means of FM1-43 experiments (2). Then, experiment analysis (3) was performed using the routine described in . Cells were then labelled with antibodies against different presynaptic proteins (4). After alignment and analysis of the acquired images (5) the immunoreactivity measurements were correlated with the data obtained in the functional experiment (6).
Ramírez-Franco et al. BMC Neuroscience 2013 14:127 doi:10.1186/1471-2202-14-127