Open Access Highly Accessed Research article

Plasticity of primary microglia on micropatterned geometries and spontaneous long-distance migration in microfluidic channels

Susanna Amadio1, Adele De Ninno23, Cinzia Montilli1, Luca Businaro2, Annamaria Gerardino2 and Cinzia Volonté1*

Author Affiliations

1 Santa Lucia Foundation/CNR-Cellular Biology and Neurobiology Institute, Via del Fosso di Fiorano 65, 00143 Rome, Italy

2 Department of Anatomy, Histology, Forensic Medicine and Orthopedics, University La Sapienza, Rome, Italy

3 CNR-Institute for Photonics and Nanotechnology, Via Cineto Romano 42, 00156 Rome, Italy

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BMC Neuroscience 2013, 14:121  doi:10.1186/1471-2202-14-121

Published: 13 October 2013

Additional files

Additional file 1:

The video shows baseline motility of primary microglia for long distances on artificial bio-mimetic microfluidic substrates with controlled physical/chemical characteristics. We performed regular 30 min time-lapse recording (JuLI, Digital Bio) over a 20 h period. Primary microglia spontaneously travel through fibronectin-coated PDMS microfluidic channels (width 12 μm and length 500 μm) for a distance of up to 500 μm in approximately 12 h, with an average speed of 0.66 μm/min.

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