The effect of Shh on Gli3 expression of motor neurons in culture. After treated with Shh, every single SMI32+ cells co-stained with Gli3 (A), but none of GFAP+ cells was found to co-stain with Gli3+ (B). Scale bar = 10 μm (A, B).
Ma et al. BMC Neuroscience 2013 14:119 doi:10.1186/1471-2202-14-119