Additional file 1: Figures S1.

Verification of OVX. The effects of OVX and the estrous stage were determined by cytological evaluations of vaginal smears under microscopic examination. The smears of rats in the control group consisted almost exclusively of leukocytes, indicating the rats were at diestrus. The smears of OVX rats also consisted of leukocytes, indicating the rats were also at diestrus, but the number of leukocytes was significantly fewer than that in the control group. Figures S2. The levels of serum estrogen were detected to confirm the HRT. After neurological evaluation, the blood was collected from the ophthalmic artery of the rats. Serum estradiol was measured by EIA kit in the control group, the OVX group, 6 μg/kg, 20 μg/kg and 50 μg/kg E2 replacement groups. the level of serum estradiol at 18.3 ± 0.7 pg/ml, 57.8 ± 8.1 pg/ml and 127.3 ± 10.4 pg/ml, which were roughly equivalent to low, high and supra physiological levels of E2. These results confirmed that HRT had achieved the desired effect. Table S1. Neurolgical evaluation after the middle cerebral artery occlusion in Wister rats. Table S2. The physiological parameters in animals of different groups before, during and after the MCAO. The physiological parameters of OVX, 6 μg/kg, 20 μg/kg and 50 μg/kg E2 group had no significant difference compared to the control group (p > 0.05).

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Ma et al. BMC Neuroscience 2013 14:118   doi:10.1186/1471-2202-14-118