The effects of different doses of E2 on cultured PC12 cell proliferation assessed by Brdu incorporation assay. (A) The triangle represents the nuclei labeled by PI dye (Red), and the arrow represents the replicating DNA by BrdU incorporation (Green). (B) The ratio of Brdu-positive cells in 10 nM and 20 nM E2 groups significantly increased (*p < 0.01, compared with the control and DMSO groups), and the Brdu-positive cells in 10 μM and 20 μM E2 group markedly decreased (#p < 0.05, v.s. the control and DMSO groups).
Ma et al. BMC Neuroscience 2013 14:118 doi:10.1186/1471-2202-14-118