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Pheromonal bile acid 3-ketopetromyzonol sulfate primes the neuroendocrine system in sea lamprey

Yu-Wen Chung-Davidson1, Huiyong Wang1, Michael J Siefkes12, Mara B Bryan13, Hong Wu14, Nicholas S Johnson15 and Weiming Li1*

Author Affiliations

1 Department of Fisheries and Wildlife, Michigan State University, 13 Natural Resources Building, 480 Wilson Road, East Lansing, MI, 48824, USA

2 Present address: Great Lakes Fishery Commission, 2100 Commonwealth Blvd., Suite 100, Ann Arbor, MI, 48105, USA

3 Present address: Energy Biosciences Institute, University of California, 130 Calvin Laboratory, MC 5230, Berkeley, CA, 94720, USA

4 Present address: Department of Microbiology & Immunology, School of Medicine, Emory University, Rollins Research Center G214, 201 Dowman Drive, Atlanta, Georgia, 30322, USA

5 Present address: USGS, Great Lakes Science Center, Hammond Bay Biological Station, 11188 Ray Road, Millersburg, MI, 49759, USA

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BMC Neuroscience 2013, 14:11  doi:10.1186/1471-2202-14-11

Published: 20 January 2013

Additional files

Additional file 1: Figure 1:

3kPZS exposure had no effect on hindbrain lGnRH-I concentrations in immature male sea lamprey. Data are presented as mean ± S.E.M. Figure 2. 3kPZS exposure had no effect on hindbrain lGnRH-III concentrations in immature male sea lamprey. Data are presented as mean ± S.E.M. Figure 3. 3kPZS exposure did not change the number of lamprey (l) GnRH-positive neurons in the hypothalamus. Lamprey (l) GnRH in situ hybridization (ISH) showed positive cells (blue stain) in the preoptic area of immature female and male sea lamprey. 20 μm Transverse sections were counterstained with nuclear fast red (pink stain). Scale bar: 20 μm. 3 V: third ventricle. Figure 4. 3kPZS exposure did not change the number of lamprey (l) GnRH-Iimmunoreactive neurons in the hypothalamus. lGnRH-I-immunoreactive neurons (red stain) are located in the preoptic area of immature female and male sea lamprey. 20 μm transverse sections were counterstained with hematoxylin (blue/purple stain). Scale bar: 50 μm. 3 V: third ventricle. Figure 5. 3kPZS exposure did not change the number of lamprey (l) GnRH-IIIimmunoreactive neurons in the hypothalamus. lGnRH-III-immunoreactive neurons (red stain) are located in the preoptic area of immature female and male sea lamprey. 20 μm transverse sections were counterstained with hematoxylin (blue/purple stain). Scale bar: 50 μm. 3 V: third ventricle.

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