Additional file 3: Figure S3.
Pipelines and image analysis. Pipeline (left) and ‘identify primary objects’ module (right) used to quantify neuronal numbers by CP software (A). Example of neuronal identification and segmentation of clumped cells (B). After inversion and transformation (right upper image), CP detects cells and segments clumped objects by staining intensity (green, right lower image).
Format: JPEG Size: 3.3MB Download file
Becker et al. BMC Neuroscience 2013 14:108 doi:10.1186/1471-2202-14-108