Additional file 2: Figure S2.
Image examples and region of interest. Example of ETNA striatal coronal section stained with pE3-Aβ specific antibody, used to quantify pE3-Aβ positive cells by CP software (A). Images were taken at 4 x magnification and automatically inverted and transformed into grayscale (B). CP software detects numbers of stained cells and staining artifacts were excluded by low staining intensity and size above 10 pixels. To quantify neuronal numbers of ETNA striatal coronal sections were stained with NeuN-specific antibody. A ROI was defined as image at 10 x magnification (1.6 mm x 0.87 mm) in the basal, lateral striatum with maximal possible distance from lateral ventricle (LV), touching the external capsule (ec) and only including cells of the striatum (C). Example of ROI used for neuronal quantification (D).
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Becker et al. BMC Neuroscience 2013 14:108 doi:10.1186/1471-2202-14-108