Effect of A-β injection in OB. A. Effect of A-β25-35 injection in OB on lipid peroxidation levels in HIPPO, OB and Frontal Cortex (FC) of ovariectomized animals. Mean ± SEM lipid peroxidation levels in the four experimental groups (24 h, 8 and 15 days and E2 replacement groups). The lipid peroxidation levels in nanomoles/ml of homogenate HIPP, OB or FC tissue depicted on the ordinate. Significant differences were found in 24 h experimental group between HIPPO and OB compared with FC tissue. Minimal amount of lipoperoxidation was detected 15 days after A-β injection in the three structures evaluated. E2 decreases the lipoperoxidation in HIPPO as well as in OB. B. Western blot technique to measure 4-HNE adduct in OB reflects the lipoperoxidation in HIPPO, OB and FC in the same experimental groups. The amount of 4-HNE adducts is related with the presence of peroxides in the structure, the administration of E2 decreases the presence of peroxides and the amount of 4-HNE.
Bernal-Mondragón et al. BMC Neuroscience 2013 14:104 doi:10.1186/1471-2202-14-104