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Open Access Highly Accessed Research article

Experience-dependent persistent expression of zif268 during rest is preserved in the aged dentate gyrus

Ali Gheidi1, Erin Azzopardi1, Allison A Adams1 and Diano F Marrone12*

Author Affiliations

1 Department of Psychology, Wilfrid Laurier University, 75 University Ave W, Waterloo, ON N2L 3C5, Canada

2 McKnight Brain Institute, University of Arizona, Tucson, AZ 85724, USA

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BMC Neuroscience 2013, 14:100  doi:10.1186/1471-2202-14-100

Published: 13 September 2013

Abstract

Background

Aging is typically accompanied by memory decline and changes in hippocampal function. Among these changes is a decline in the activity of the dentate gyrus (DG) during behavior. Lasting memory, however, is thought to also require recapitulation of recent memory traces during subsequent rest – a phenomenon, termed memory trace reactivation, which is compromised in hippocampal CA1 with progressive age. This process has yet to be assessed in the aged DG, despite its prominent role in age-related memory impairment. Using zif268 transcription to measure granule cell recruitment, DG activity in adult and aged animals was assessed both during spatial exploration and as animals remained at rest in the home cage in order to detect potential memory-related replay.

Results

Consistent with the observation of memory trace reactivation in DG, the probability that an individual granule cell transcribes zif268 during rest in the animal’s home cage is increased by recent experience in a novel environment. Surprisingly, a comparable increase was observed in the probability of granule cells in the aged DG expressing zif268 during rest. Moreover, no significant age-related difference was observed in the number of granule cells expressing zif268 during rest. Thus, the number and pattern of granule cell expression of zif268 during rest is preserved in aged animals, despite a significant decline in exploration-related zif268 expression.

Conclusions

These data lead to the hypothesis that the input the aged DG receives from backprojections from CA3 (the region widely hypothesized to mediate reactivation) remains functionally intact despite loss of innervation from the perforant path.

Keywords:
Fascia dentata; egr1; ngfi-a; Reactivation; Replay; Granule cell; IEG; Hippocampus