Temporal curve of MKP-7 protein levels and activities during different reperfusion times following cerebral ischemia. Proteins were extracted and measured using antibodies against MKP-7. MKP-7 was purified by immunoprecipitation, and its activity was measured using an alkaline phosphatase assay. A: Time curve of MKP-7 levels during different reperfusion times (15 min, 1, 2, 4, 6 and 24 h) following 10 min of ischemia. B: Temporal curve of MKP-7 activity after different reperfusion times (15 min, 1, 2, 4, 6 and 24 h) following 10 min of ischemia. OD data are presented as means ± SEM (n = 4) and expressed as magnitudes of the alteration versus sham control or control [*P < 0.05 versus sham control].
Zhu et al. BMC Neuroscience 2013 14:1 doi:10.1186/1471-2202-14-1