Western blot analysis of JNK, Akt, SEK1 and MKK-7 activities in response to ischemia at different times after reperfusion and effect of PI3K/Akt inhibitor LY294002 after 1 h of reperfusion. Proteins were extracted and measured using antibodies against p-JNK (Thr183/Tyr185), p-Akt (Ser473), p-MKK-7 (Ser271/Thr275), p-SEK1 (Ser80), JNK, Akt, MKK-7 and SEK1. A: Phosphorylation state of JNK, Akt, MKK-7 and SEK1 after different reperfusion times (15 min, 1, 2, 4, 6 and 24 h) following 10 min of ischemia. B: Determination of p-JNK, p-MKK-7 and p-SEK1 levels in animals administered LY294002 (LY) compared with sham and control groups (vehicle, Veh). ODs are presented as means ± SEM (n = 4) and expressed as magnitudes of change versus sham control or control. *P < 0.05. versus sham control; #P < 0.05, comparison between the 1-h chemically treated reperfusion groups and their respective vehicle controls.
Zhu et al. BMC Neuroscience 2013 14:1 doi:10.1186/1471-2202-14-1