Additional file 2.
Figure S2. Acute survival of STROC05 in WT mice. An injection of 225,000 STROC05 cells in 3 μl (75,000 cells/μl) at 7 weeks of age into wild-type mice resulted in a good graft survival (Human nuclei antigen, HNA, in red, DAPI in blue), even in the absence of immunosuppression. Cells remained within the injection tract and did not exhibit any migration out of their site of injection. To ensure a better distribution of cells within the striatum, two deposits were placed within the same injection tract. A glial reaction (GFAP + cells in green) was evident along the injection tract. These results indicate that STROC05 cells can survive in WT animals and that using this protocol there is a robust engraftment.
Format: JPEG Size: 3MB Download file
El-Akabawy et al. BMC Neuroscience 2012 13:97 doi:10.1186/1471-2202-13-97