Additional file 1.
Figure S1. STROC05 survival in the 3-nitroproprionic acid (3-NPA) rat model of Huntington’s disease. Male Lewis rats (220-250 g) received i.p. injections of 42 mg/kg 3-NPA. (Sigma-Aldrich) for five consecutive days to induce a bilateral degeneration of striatal cells, as previously described . Animals gradually develop a behavioural phenotype and show a progressive striatal tissue loss that coincides with neuronal loss, as well as an increase in glial scarring and microglia activity . Additionally, these animals show a clear deficit in brain activity [7,60]. Two weeks after lesion induction, animals received unilateral injections of 400,000 STROC05 human neural stem cells (hNSCs). hNSCs can be detected in the injection tract using human nuclear antigen (A). The presence of CD11b + microglia reveals the inflammatory response to the ongoing neurodegeneration in the lateral striatum and indicates a placement of cells just peripheral to the damage. Higher magnification images reveal a limited migration from the injection tract to the area of damage (B&C). STROC05 cells retained some expression of nestin (D&E), but also partially differentiated into GFAP + astrocytes. Using brightfield microscopy of cell survival (G) in animals that were either immunocompetent or immunosuppressed using Cyclosporine A (CsA, Sandimmun, Novartis, 10 mg/kg, diluted in Ringer’s solution) and methylpredinolone (20 mg/kg day 1–7; 10 mg/kg day 8–12; 5 mg/kg day 13–14 i.p., Pharmacia Upjohn), a sterelogical analysis indicated a robust cell survival under both conditions over 90 days. Over 10,000 cells survived in the immunocompetent group and 25,000 cells were present in the immunosuppressed rats. It was only at 90 days survival that there was a significant difference between immunosuppression and immunocompetent animals (* P < .05), but there was no significant decrease in cell number between 30 and 90 days. Discontinuation of immunosuppression also did not lead to a graft rejection.
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El-Akabawy et al. BMC Neuroscience 2012 13:97 doi:10.1186/1471-2202-13-97