Survival of the transplanted cells. A small population of implanted cells survived (A). Human cells (human nuclear antigen + cells in pink) were mostly found within the injection tract in the striatum. A select few individual cells were observed migrating along the corpus callosum. Stereological cell counts revealed no significant difference between undifferentiated and differentiated cell implantation (B). However, cell survival was very variable with some animals having no surviving cells. Neuronal differentiation, as determined by FOX3 staining, of implanted cells was very poor (C&D). Most implanted cells differentiated into astrocytes (E), whereas others did neither express markers of neurons nor astrocytes. (Scale bar 200 μM).
El-Akabawy et al. BMC Neuroscience 2012 13:97 doi:10.1186/1471-2202-13-97