Figure 3 .
PP2 rather acts on SC proliferation and survival than on direct SC migration.A: Scheme showing the experimental setup (Expl. = Explantation, Analy. = Analysis, DIV = Day In Vitro). B/C: Immunohistochemical stainings for TH (green) and pHH3 (red) with DAPI nuclear counterlabeling of control (B) and PP2 treated (C) SCG explants. Scale bars = 200 μm. Please note the reduced number of pHH3 positive cells (white arrows) after PP2 treatment in comparison to the control. D/E: close up of ctr (D) and PP2 treated (E) SCs costained with DAPI nuclear labeling and pHH3. One can appreciate the increased level of fragmented nuclei after PP2 treatment. F: Quantitative analyzes of SC migration distances in %. (students t test: p value = 0.0508) No significant difference in migration distances was observed when PP2 was added at DIV3. G: Quantitative analyzes of Proliferation using the pHH3/DAPI ratio in %. A strongly reduced level of mitosis was observed, when PP2 was added at DIV3 (students t test: p value < 0.0001).
Heermann et al. BMC Neuroscience 2012 13:92 doi:10.1186/1471-2202-13-92