RORα and SOD1 reduce ATXN7Q65-Myc protein aggregation. A) HEK 293 cells were co-transfected to express ATXN7Q65-Myc and RORα. After 48 hours RORα and ATXN7Q65-Myc expression (left panel) and ATXN7 aggregation (right panel) were analyzed and quantified. Actin was used as loading control for western blots. B) HEK 293 T cells were co-transfected with ATXN7Q65-Myc and 0–1 μg of a plasmid encoding wild-type (WT), A4V mutant or H48Q mutant SOD1. Empty vector (Myc) was used to allow the same amount of plasmids to be transfected in each well. Forty-eight hours after transfection ATXN7Q65-Myc aggregation was analyzed by filter trap in cells co-transfected with WT SOD1 (left), A4V SOD1 (middle) and H48Q SOD1 (right). All quantifications are shown as means ± SEM from three independent experiments with triplicates. NS: not significant, * p <0.05 and ** p <0.01.
Ajayi et al. BMC Neuroscience 2012 13:86 doi:10.1186/1471-2202-13-86