Figure 2.

Antioxidant treatment rescues cells from ATXN7Q65-GFP induced toxicity and decrease ATXN7 aggregation. A) Viability measured by WST-1 in FLQ65 cells not induced (+Dox) or induced (−Dox) to express ATXN7Q65-GFP for 9 days while growing in media with or without 1 μM Vitamin E (α-tocopherol). B) Analysis and quantification of soluble ATXN7Q65-GFP by western blot in FLQ65 cells grown and treated as in A. C) Analysis and quantification of ATXN7 aggregation by filter trap assay in FLQ65 cells grown and treated as in A. D) Viability in cells grown as in A but treated with or without 5 mM NAC. E) Analysis and quantification of soluble ATXN7Q65-GFP by western blot in FLQ65 cells grown and treated as in D. F) Analysis and quantification of ATXN7 aggregation by filter trap assay in FLQ65 cells grown and treated as in D. For all western blots actin was used as loading control. For quantifications data are shown as means ± SEM from three independent experiments with triplicates. NS: not significant, * p <0.05, ** p <0.01 and *** p <0.001.

Ajayi et al. BMC Neuroscience 2012 13:86   doi:10.1186/1471-2202-13-86
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