Impact of AdOx on H3K27me3, H3K9me3 & H3K9me2 marks on the nanog and oct3/4 genes in P19 cells. A. P19 cells were treated with the indicated concentrations of AdOx, and the nuclear extracts were analyzed by Western blot with the indicated antibodies for specific histone methylation marks. Numbers indicated quantification of the H3K27me3, K9me3, or K9me2 western signal relative to loading respectively. Digits at the bottom of the top three rows indicates the relative darkness of each band compared with the control lane set as 1.0. B. Positions of the primer pairs used in ChIP assays for analyzing the repressive methylation marks on the nanog and oct3/4 genes. C & D. Effect of AdOx on H3K27me3, H3K9me3, or H3K9me2 levels on the nanog (C) and oct3/4 (D) genes in P19 cells. The positions of the methylated histone marks to the genes are indicated at the bottom of each group shown in ChIP assays. IgG is a negative antibody control. Annotations are as described in Figure 1G.
Yan et al. BMC Neuroscience 2012 13:6 doi:10.1186/1471-2202-13-6