Double-label confocal images showing P2X2- with syntaxin-1- or 5-HT-LIR in rat circumvallate taste buds. A: P2X2-LIR nerve processes extend from basal lamina to taste pore. B: A subset of taste cells (arrowheads) and nerve processes in taste buds displaying syntaxin-1-LIR. C: P2X2-LIR nerve processes also express syntaxin-1 (arrow). D: P2X2-LIR. E: 5-HT is present in a subset of taste cells (arrowheads). No colocalization occurs between P2X2- and 5-HT-LIR in the taste buds, however, P2X2 immunoreactive nerve processes are in close apposition with 5-HT-LIR cells (e.g., rectangular box). TP: taste pore; BL: basal lamina. Scale bars = 20 μm.
Yang et al. BMC Neuroscience 2012 13:51 doi:10.1186/1471-2202-13-51