Table 1

Phenotype of newborn cells 28 days after BrdU delivery
BrdU NeuN GFAP
WT KO WT KO WT KO
mean ± SEM mean ± SEM mean ± SEM mean ± SEM mean ± SEM mean ± SEM
P7 (n = 8) (n = 3)
BrdU abs. 62466.0 ± 3239.3 22394.0 ± 1455.3*** --- ± --- --- ± --- --- ± --- --- ± ---
% of BrdU+ --- ± --- --- ± --- 57.4 ± 2.0 60.7 ± 0.3 6.3 ± 1.9 4.9 ± 1.2
abs. co-lab. --- ± --- --- ± --- 35621.2 ± 1643.7 13593.5 ± 845.7** 3831.5 ± 1060.3 1140.2 ± 336.9**
P14 (n = 5) (n = 4)
BrdU abs. 13147.2 ± 1060.1 5671.5 ± 798.5** --- ± --- --- ± --- --- ± --- --- ± ---
% of BrdU+ --- ± --- --- ± --- 64.7 ± 2.2 51.0 ± 5.2 1.9 ± 1.2 1.2 ± 0.8
abs. co-lab. --- ± --- --- ± --- 8480.3 ± 649.6 2812.2 ± 271.3** 223.0 ± 141.4 84.3 ± 63.7
P28 (n = 4) (n = 3)
BrdU abs. 5578.5 ± 708.4 390.0 ± 104.3*** --- ± --- --- ± --- --- ± --- --- ± ---
% of BrdU+ --- ± --- --- ± --- 73.1 ± 3.8 64.8 ± 2.6 0.9 ± 0.1 2.6 ± 2.6
abs. co-lab. --- ± --- --- ± --- 4100.7 ± 583.4 261.1 ± 88.5*** 45.8 ± 6.6 6.0 ± 6.0
P40 (n = 3) (n = 3)
BrdU abs. 3726.0 ± 305.2 306.0 ± 51.3*** --- ± --- --- ± --- --- ± --- --- ± ---
% of BrdU+ --- ± --- --- ± --- 64.6 ± 4.0 60.2 ± 7.1 8.3 ± 4.5 12.2 ± 6.5
abs. co-lab. --- ± --- --- ± --- 2420.3 ± 299.7 190.7 ± 53.1*** 313.5 ± 186.6 31.3 ± 17.0
P60 (n = 4) (n = 4)
BrdU abs. 2893.5 ± 205.5 222.0 ± 52.7*** --- ± --- --- ± --- --- ± --- --- ± ---
% of BrdU+ --- ± --- --- ± --- 60.1 ± 4.1 46.3 ± 5.7 3.8 ± 2.8 0.0 ± 0.0
abs. co-lab. --- ± --- --- ± --- 1756.7 ± 219.8 101.3 ± 23.6*** 94.9 ± 68.6 0.0 ± 0.0
P90 (n = 4) (n = 4)
BrdU abs. 1770.0 ± 354.2 205.5 ± 81.5*** --- ± --- --- ± --- --- ± --- --- ± ---
% of BrdU+ --- ± --- --- ± --- 66.6 ± 6.6 72.5 ± 14.8 5.7 ± 1.5 10.8 ± 7.9
abs. co-lab. --- ± --- --- ± --- 1136.2 ± 198.5 157.9 ± 79.2*** 107.0 ± 30.1 29.7 ± 18.4
P260 (n = 4) (n = 3)
BrdU abs. 300.0 ± 60.1 26.0 ± 6.2*** --- ± --- --- ± --- --- ± --- --- ± ---
% of BrdU+ --- ± --- --- ± --- 49.7 ± 2.0 44.4 ± 29.4 9.1 ± 3.2 5.6 ± 5.6
abs. co-lab. --- ± --- --- ± --- 150.0 ± 33.5 13.3 ± 11.4*** 30.1 ± 15.4 0.7 ± 0.7

Two-way ANOVA on ln-transformed data (*p < 0.05, **p < 0.01, ***p < 0.001 cD2KO compared to WT); Abbreviations: abs. – absolute, abs. co-lab. – absolute number of cells co-labeled with BrdU.

Ansorg et al.

Ansorg et al. BMC Neuroscience 2012 13:46   doi:10.1186/1471-2202-13-46

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