Figure 3.

Pharmacology of CCh effect on paired-pulse stimulation of field potentials. A, B, C. Example of field potential recordings using a paired-pulse protocol in control (A), 10 μM CCh (B) and during 10 μM CCh + 1 μM atropine co-application (C). Note that 10 μM CCh decreased the paired pulse depression compared to control condition. Co-application of 1 μM atropine with 10 μM CCh reversed this effect. D. Plots of the paired-pulse ratio (PPR) at different interstimulus intervals (10, 20 and 30 ms), in control and in the presence of 10 μM CCh (n = 49). E - H. The effects of different mAChR antagonists and of 10 μM linopirdine on CCh-induced alterations of paired-pulse ratio, as indicated. Note that co-application of 1 μM atropine (E; n = 19) or 2 μM AFDX (F; n = 15) with CCh reversed the CCh-mediated increase of PPR, whereas 1 μM pirenzepine (G; n = 14) had little effects. Note that linopirdine (H; n = 10) did not mimick the CCh-induced increase of PPR. These experiments were performed in an ITRC. The symbols represent: *: p < 0.05 vs. control, **: p < 0.01 vs. control, ***: p < 0.001 vs. control; +: p < 0.05 vs. CCh, ++: p < 0.01 vs. CCh, +++: p < 0.001 vs. CCh

Gigout et al. BMC Neuroscience 2012 13:42   doi:10.1186/1471-2202-13-42
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