Additional file 2.
Figure S2 Effects of β-BuTX on voltage-dependent Ca2+ channels. A: Currents through voltage-dependent Ca2+ channels were measured under extra- and intracellular K+-free conditions in the presence of 10 mM Ba2+ as a charge carrier and 10 nM TTX to block Na+ channel currents. The cells were electrically stimulated by 10 depolarizing voltage-steps of 50 ms duration with 10 mV increasing amplitude from a holding potential of -70 mV. B: In the presence of Ba2+ the electrical stimulation results in fast activating and slowly inactivating inward currents. C: Current/voltage plot showing the effects of 10 pM β-BuTX on Ba2+ currents. The currents activated at -40 mV and reached maximal current amplitude at 0 mV. The currents were inhibited by 20% after application of 10 μM (+)BayK8644 indicting that several types of high-voltage activated Ca2+ channels contribute to the Ba2+ currents. Application of β-BuTX did change neither current density nor voltage-dependence of the Ba2+ currents.
Format: PDF Size: 79KB Download file
This file can be viewed with: Adobe Acrobat Reader
Guo et al. BMC Neuroscience 2012 13:36 doi:10.1186/1471-2202-13-36