Expression of VEGF in RPE/choroid of mice. (A-C) Immunohistochemistry using a specific antibody for VEGF visualized by 3-Amino-9-ethylcarbazole (AEC, red) staining and microscopy reveals increased immunoreactivity of VEGF in the region of outer retina in mice received CILE (C) compared with both non-transgenic (non-Tg) and the bigenic control (B). Scale bar = 20 μm. (D) Quantification of VEGF immunoreactivity Bars depict mean ± SEM. *: P < 0.001 (N = 6). (E) Western blots using the same antibody for VEGF confirms increased immunoreactivity of VEGF in the retina of mice following 6-month CILE.
Dong et al. BMC Neuroscience 2012 13:34 doi:10.1186/1471-2202-13-34