Transmission electron microscopic analysis of retina. Transmission electron microscopy was conducted as described in the Methods. Representative micrographs covering the RPE regions are shown here. (A) Normal morphology of photoreceptor outer segment (OS), RPE and Bruch's membrane (BrM) is detected in non-Tg control mouse. (B). APP/PS1 bigenic control mouse retina demonstrates slight degenerating changes in the RPE but with normal morphology of Bruch's membrane. (C-J) In the retina from the group of bigenic mice following 6-month CILE (light-6 m), micrographs show massive vacuoles in the RPE cells and disrupted photoreceptor outer segments (C), vacuoles in RPE cells at a higher magnification (D), degenerative changes with small basal laminar deposits in RPE (E, arrowhead, dashed line enclosure), abnormal appearance of basal infoldings (G, arrowhead), breaks of the elastic layer of Bruch's membrane (G, open arrowheads), and thickened or distorted Bruch's membrane (BrM) in RPE cells (F&G). Bold arrows in (D-J) indicate endothelial fenestrations of choriocapillaris (CC) and infiltration of microglia (G&I, thin arrows) is seen in the RPE-Bruch's membrane interface. (H) The outer collagenous layer (OCL) is observed adjacent to Bruch's membrane. (I) Neovascularization (NV) and adjacent capillary (CC) are found on both sides of Bruch's membrane. An image from an adjacent section to the boxed area in (I) is shown in (J) at a higher magnification. Scale bar = 4 μm forA, B, H, I, 2 μm for E, 1 μm for, 3 μm for C, G, 1.5 μm for D, F, and J.
Dong et al. BMC Neuroscience 2012 13:34 doi:10.1186/1471-2202-13-34