Concentration and serum preincubation dependence of Cai++ release by 5-HT and DOI in CHO cells expressing 5-HT2AR (A, B) or 5-HT2CR (C, D). Cells plated at 16,000 cells/well in 96-well plates for 24 hrs were either preincubated overnight in serum-free (open circles) or in serum-replete (closed circles) medium and loaded with Calcium 4. Peak height following stimulation was recorded for each well and normalized to the maximal response to 5-HT. n = 5-6; * p < 0.05, *** p < 0.001 vs. respective serum preincubation.
Seitz et al. BMC Neuroscience 2012 13:25 doi:10.1186/1471-2202-13-25