Table 2

The CUS procedure

Time

Course of the experiment


The 1st day 9:00

cage rocking (5 times per second) for 15 min


The 2nd day 9:00

swimming in cold (4°C) water for 5 min


The 3rd day 9:00

soiled cage (250 ml of tap water into the sawdust

bedding) for 24 h


The 4th day 9:00

lights on overnight


The 5th day 9:00

cage tilting for 24 h


The 6th day 9:00

restraint for 12 h


The 7th day 8:00

food and water deprivation for 24 h


The 8th day 8:00

one-bottled-sucrose test sessions (last 1 h)


9:00

soiled cage for 24 h


The 9th day 9:00

electric stimulus (1.0 mA, each time for 1 second,

10 times per minute) for 5 min


The 10th day 9:00

elevated temperature (40°C) for 15 min


The 11th day 9:00

cage rocking for 15 min


The 12th day 9:00

lights on overnight


The 13th day 9:00

restraint for 12 h


The 14th day 8:00

food and water deprivation for 24 h


The 15th day 8:00

one-bottled-sucrose test sessions (last 1 h)


9:00

lights on overnight


The 16th day 9:00

swimming in cold water for 5 min


The 17th day 9:00

restraint for 12 h


The 18th day 9:00

electric stimulus for 5 min


The 19th day 9:00

cage rocking for 15 min


The 20th day 9:00

soiled cage for 24 h


The 21th day 9:00

cage tilting for 24 h (The CUS procedure finish)


The 22th day 6:30

weighed body weight and open field test


8:00

food and water deprivation for 24 h


The 23th day 8:00

one-bottled-sucrose test sessions (last 1 h)


Huang et al. BMC Neuroscience 2012 13:22   doi:10.1186/1471-2202-13-22

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