(A) MAOB microarray mRNA data reveals the steep rise in expression with aging (regression p < 0.001); (B) qPCR mRNA results display similar pattern of up-regulation with age (p = 0.001). Post hoc analysis revealed the adult group possessed significantly higher mRNA compared to the neonate (p = 0.002), infant, toddler (both p < 0.001), and adolescent (p = 0.014) age groups. Young adults also had greater MAOB mRNA compared to the neonates (p = 0.023), infants (p = 0.007), and toddlers (p = 0.009) while the school age group had significantly higher mRNA than infants (p = 0.041) and toddlers (p = 0.045). (C) A representative Western blot indicating the identifying band of MAOB protein at 60 kDa in most age groups. N = Neonate, I = Infant, T = Toddler, Y = Young Adult, S = School Age, A = Adult, Ad = Adolescent. (D) MOAB protein expression peaks in adolescence (p < 0.001) and is much more abundant in both young adults and adults compared to the neonate (p < 0.001), infant (p < 0.01), and toddler (p ≤ 0.01) age groups. ** p ≤ 0.01, * p ≤ 0.05.
Rothmond et al. BMC Neuroscience 2012 13:18 doi:10.1186/1471-2202-13-18