(A) MAO-A microarray mRNA expression significantly changed with age (regression p < 0.001) with school age having the lowest mRNA levels; (B) Post hoc analysis of qPCR mRNA data confirmed that the school age group had the lowest expression (p < 0.001) and along with the young adult (p < 0.001) age group had reduced mRNA compared to the neonate group. (C) A representative Western blot with the expected 61 kDa band for MAOA protein, and additional band at ~ 52 kDa. I = Infant, N = Neonate, T = Toddler, S = School Age, Ad = Adolescent, Y = Young Adult, A = Adult. (D) There is a dramatic difference in protein expression between age groups and opposite to that of MAOA mRNA. High immunoreactivity was observed in the older age groups, with young adults having significantly greater MAOA protein compared to neonates, infants, toddlers, school age groups (p < 0.001) as did the adolescents and adults (p < 0.01). *** p ≤ 0.001, ** p ≤ 0.01, * p ≤ 0.05.
Rothmond et al. BMC Neuroscience 2012 13:18 doi:10.1186/1471-2202-13-18