(A) DRD1 microarray (p = 0.002) and (B) qPCR mRNA results show a similar pattern of increasing expression with age (p = 0.037) until adulthood. (C) A representative Western blot for 6 of 7 age groups with the expected protein band at ~49 kDa. A = Adult, N = Neonate, S = School Age, Y = Young Adult, T = Toddler. (D) Protein expression increased robustly with age and appears to plateau during early adulthood. Adults and young adults had the highest levels of protein compared to neonates (both p < 0.001) infants (both p < 0.001), toddlers (p = 0.004, p = 0.006), and school age (p = 0.012, p = 0.009). Adolescents had greater levels of DRD1 protein than neonates (p < 0.001) and infants (p = 0.004). ** p ≤ 0.01, * p ≤ 0.05.
Rothmond et al. BMC Neuroscience 2012 13:18 doi:10.1186/1471-2202-13-18