Table 1

Proteomic profile of micriglial-derived exosomes induced by Wnt3a treatment
Protein MW (Da) Peptides matched Function
Laminin subunit beta-1 196776 1 Basement membrane
Clathrin heavychain 1 191477 9 Membrane trafficing
Laminin subunit gamma-1 177185 2 Basement membrane
Alpha-1-macroglobulin 167019 1 Inhibits proteases
Peroxidasin homolog 165029 16 ECM peroxidase (basement membrane)
Nidogen-1 136536 1 Basement membrane
Adipocyte enhancer-building protein 1 130847 4 Transcriptional repressor (cholesterol metabolism)
Major vault protein 95739 5 Drug resistance
Transitional endoplasmatic reticulum ATPase 89293 8 Budding of vesicels from ER
Integrin beta-2 84970 2 Cell adhesion
Heat shock protein HS 90-alpha 84762 1 Chaperone (folding of cyctolic proteins)
V-type proton ATPases catalytic subunit A 68283 1 Proton pump
Transketolase 67601 1 Cytosolic metabolic enzyme
Galectin-3-binding protein 63701 1 Cell adhesion via integrins
Dihydropyrimidinase-related protein 62157 1 CRMP (schizophrenia risk gene) development
ATP synthase subunit alpha, mitochondrial 59717 3 Mitochondrial enzyme
T-complex protein 1 subunit eta 59614 1 Chaperone (folding of cytosolic proteins)
Pyruvate kinase isozymes M1/M2 57781 7 Glycolysis (cytosol)
T-complex protein 1 subunit beta 57422 1 Chaperone (folding of cytosolic proteins)
Matrix metalloproteinase-19 57321 2 Secreted protease (ECMremodelling)
Cystosol aminopeptidase 56115 1 Protein turnover
Vitronectin 54271 1 Extracellular matrix
Lipoprotein lipase 53049 2 Lipid hydrolysis
Tubulin alpha-1B chain 50120 3 Structural
Tubulin beta-5 chain 49639 4 Structural
Actin, cytoplasmic 2 41766 28 Structural
Actin, cytoplasmic 1 41710 3 Structural
Protein Wnt-3a 39232 15 Treatment
Annexin A2 38654 2 Structural/ inhibits phospolipase A2 (anti inflam)/Apoptosis
Annexin A3 36341 9 Structural/ inhibits phospolipase A2 (anti inflam)/Apoptosis
Annexin A4 35826 2 Structural/ inhibits phospolipase A2 (anti inflam)/Apoptosis
Apolipoprotein E 35731 10 Lipoprotein
Glyceraldehyde-3-phosphate dehydrogenase 35725 8 Glycolysis(cytosol)
Annexin A5 35722 1 Structural/ inhibits phospolipase A2 (anti inflam)/Apoptosis
40S ribosomal protein SA 32803 3 Protein synthesis
40S ribosomal protein SA 32803 1 Protein synthesis
40S ribosomal protein S3a 29926 1 Protein synthesis
Proteasome subunit beta type-7 29908 2 Proteasomal degradation
40S ribosomal protein S3 26657 5 Protein synthesis
Proteinsome subunit alpha type-2 25910 1 Proteasomal degradation
40S ribosomal protein S8 24190 2 Protein synthesis
40S ribosomal protein S5 22864 1 Protein synthesis
Ferritin heavy chain 21113 2 Intracellular iron storage
Ferritin light chain 1 20736 10 Intracellular iron storage
Ubiquitin 8560 1 Proteasomal degradation

Microglia (2500000 cells) were treated with Wnt3a (10 nM) for 8 hours in SFM. Cell culture medium was collected, centrifuged at 10000xg for 10 minutes, concentrated using 3kDa centrifugal devices and exosomes were isolated at 100000xg. Exosomal proteins were separated by SDS-PAGE (10)% and visualised using coomassie blue. The exosomal proteome was subsequently interrogated using LC/MS/MS and database searching. Proteins are listed according to their molecular weight and the number of peptides matched per protein is presented along with protein function.

Hooper et al.

Hooper et al. BMC Neuroscience 2012 13:144   doi:10.1186/1471-2202-13-144

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