Table 1

List of primary antibodies used for immunofluorescence and immunohistochemical analysis
Antibodies Cell type Origin Company Dilution Antibody pre-treatment Abbreviation
anti-ASH1 (MASH1) neurogenic precursors rabbit polyclonal Millipore 1:20 Citrate buffer Mash1
anti-bromodeoxyuridine proliferative cells mouse monoclonal Millipore 1:50 HCl BrdU
anti-carnosine immature and mature OSNs rabbit polyclonal Millipore 1:50 TE buffer carnosine
anti-CD15 glandular cells mouse monoclonal BD Biosciences 1:10 Citrate buffer CD15
anti-glial fibrillary acidic protein olfactory ensheathing glia rabbit polyclonal Dako 1:250 nil GFAP
anti-keratin HBCs rabbit polyclonal Dako 1:200 nil keratin
anti-Ki-67, clone SP6 proliferative cells rabbit monoclonal Thermo Scientific 1:50 nil Ki67
anti-olfactory marker protein mature OSNs goat polyclonal Santa Cruz Biotechnology 1:800 TE buffer OMP
anti-Pax6, C-terminus neurogenic precursors rabbit polyclonal Millipore 1:200 Citrate buffer Pax6
anti-phospho-Histone H3 [pSer10] mitotic marker rabbit polyclonal Sigma 1:200 nil pH3
anti-╬▓III Tubulin immature and mature OSNs mouse monoclonal Promega 1:800 nil ╬▓IIITubulin

Cunha et al.

Cunha et al. BMC Neuroscience 2012 13:134   doi:10.1186/1471-2202-13-134

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