mRNA, protein and activity of cPLA2and phosphorylated (phospho)-cPLA2protein in brain of rats fed the control or high-sucrose diet for 8 weeks.A) cPLA2 (IVA) mRNA, B) cPLA2 (IVA) protein, C) phospho- cPLA2 (IVA) protein and D) cPLA2 activity. Data are expressed as the relative level of the cPLA2 normalized to the endogenous control (β-globulin) using the ΔΔCT method. Protein level is the ratio of optical density of cPLA2 to β–actin, expressed as percent of control. Values are mean ± SD (n = 8 for both groups). *p < 0.05, **p < 0.01 by unpaired t-test.
Taha et al. BMC Neuroscience 2012 13:131 doi:10.1186/1471-2202-13-131