Rotary conditions increase NPC yield during differentiation. A. Bright-field images of ESCs differentiated under static (left) or rotary conditions (right; 45 rpm). Rotary conditions produced spherical aggregates without agglomeration. B. Average NPC yields for each cell line at DIV 0. Rotary culture of cell aggregates improves neural progenitor cell recovery approximately three-fold (P < .001, n=5).
Hubbard et al. BMC Neuroscience 2012 13:127 doi:10.1186/1471-2202-13-127