Suspension-adapted ESNs remain mitotically stable and express markers of pluripotency. A. ESC yields during the first five passages after transition to suspension culture. B. Summary of flow cytometry data demonstrating no substantive change in Oct3/4 expression in three different mESC lines across 25 passages in suspension culture (n=6 for each ESC line). LIF indicates leukocyte inhibitor factor. A, B. Error bars indicate standard deviation. C. Black, actual yields for a representative R1 ESC culture between 5–30 passages after suspension adaptation. Theoretical yields of ESCs (red, filled) and NPCs (red, not filled) over 25 passages are also presented.
Hubbard et al. BMC Neuroscience 2012 13:127 doi:10.1186/1471-2202-13-127