Additional file 1.
Figure S1. Increase 9cRA level in brain after intranasal delivery of 9cRA. A total of 12 rats were given either 9cRA (20 ug per animal) or vehicle intranasally. Brain tissues were harvested at 1, 2 and 4 hours after administration. Tissues were extracted and 9cRA levels in brain homogenates were detected by Mass spectrometry as previously described (Kane, A. Biochem J. 388,363-369, 2005). No detectable 9cRA was found in vehicle treated rats. There is a significant increase in 9cRA level at one and 2 hours after 9cRA administration (p<0.05, one way ANOVA). Brain 9cRA level returned to the basal level at 4 hours after administration.
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Yin et al. BMC Neuroscience 2012 13:120 doi:10.1186/1471-2202-13-120