p-cPLA2 and beta III tubulin colocalise in neurons after HuPrP106-126 treatment and is inhibited by PACOCF3. (A) Primary cortical neurons were untreated, treated with 40 μM HuPrP106-126, HuPrP106-126scram, or pre-treated with 1 μM PACOCF3 prior to addition of peptide. p-cPLA2 and beta III tubulin was analyzed using anti-p-cPLA2 (red) and anti-beta III tubulin (green) antibodies. Nuclei were stained with DAPI. Images show a representative picture of three individual experiments. Scale bar: 50 μM (B) Colocalisation of the two antibodies was measured by correlation. An overall average colocalisation of all results is shown. ***P < 0.001.
Last et al. BMC Neuroscience 2012 13:106 doi:10.1186/1471-2202-13-106