HuPrP106-126 causes cPLA2 activation and relocation in primary cortical neurons. (A) CD-1 murine primary cortical neurons of an in vivo age of 7 days were treated for 0 (left panel) or 30 minutes (right panel) with 40 μM HuPrP106-126 or HuPrP106-126 scrambled peptides, or 1 μM PMA + 5 μM A23187. The cellular localization of p-cPLA2 was analyzed by immunofluorescence using anti-p-cPLA2 (red) antibody. In addition actin was visualised in PMA + A23187 samples using phalloidin (green). Nuclei were stained with DAPI. Scale bar: 50 μM. Intensity of p-cPLA2 labelling was measured and normalised to cell number, shown in accompanying graph. (B) PrP null murine primary cortical neurons were treated with 40 μM HuPrP106-126, HuPrP106-126 scrambled or 1 μM PMA + 5 μM A23187. Intensity of p-cPLA2 labelling was measured and normalised to cell number. Data expressed as mean ± S.D. of three experiments. *P < 0.05, ***P < 0.001.
Last et al. BMC Neuroscience 2012 13:106 doi:10.1186/1471-2202-13-106