Figure 3.

In vivo assessment of the permeability of the BBB permeability to ZMB034 and ZMJ018 administered by intracardiac injection. ZMB034 or ZMJ018 was injected into the cardiac chamber of zebrafish larvae (MK001 line) at 7–8 dpf. In vivo imaging of zebrafish brain was performed using a CLSM. A-D: In vivo fluorescence imaging of zebrafish larvae injected with ZMB034 (A and C) or ZMJ018 (B and D) at 10 min (A and B) and 30 min (C and D) post-injection. While the CBV were clearly visualized in zebrafish injected with ZMB034 or ZMJ018, the OT was visualized only in zebrafish treated with ZMB034. E: Quantitative analysis of FI of the OT relative to the FI of CBV. The relative FI at 10 and 30 min after intracardiac injection was significantly higher in zebrafish injected with ZMB034 than in those injected with ZMJ018 (n = 4, *P < 0.05). Scale bar: 50 μm. FI, fluorescence intensity.