Serotonin-mediated modulation of Na+/K+ pump current in rat hippocampal CA1 pyramidal neurons
1 Department of Pharmacology, Hebei Medical University, 361 Zhongshan East Road, Shijiazhuang, 050017, China
2 Department of Pharmacy, College of Chemical and Pharmaceutical Engineering, Hebei University of Science and Technology, 70 Yuhua East Road, Shijiazhuang, 050018, China
3 Department of Toxicology, Hebei Medical University, 361 Zhongshan East Road, Shijiazhuang, 050017, China
BMC Neuroscience 2012, 13:10 doi:10.1186/1471-2202-13-10Published: 19 January 2012
The aim of this study was to investigate whether serotonin (5-hydroxytryptamine, 5-HT) can modulate Na+/K+ pump in rat hippocampal CA1 pyramidal neurons.
5-HT (0.1, 1 mM) showed Na+/K+ pump current (Ip) densities of 0.40 ± 0.04, 0.34 ± 0.03 pA/pF contrast to 0.63 ± 0.04 pA/pF of the control of 0.5 mM strophanthidin (Str), demonstrating 5-HT-induced inhibition of Ip in a dose-dependent manner in hippocampal CA1 pyramidal neurons. The effect was partly attenuated by ondasetron, a 5-HT3 receptor (5-HT3R) antagonist, not by WAY100635, a 5-HT1AR antagonist, while 1-(3-Chlorophenyl) biguanide hydrochloride (m-CPBG), a 5-HT3R specific agonist, mimicked the effect of 5-HT on Ip.
5-HT inhibits neuronal Na+/K+ pump activity via 5-HT3R in rat hippocampal CA1 pyramidal neurons. This discloses novel mechanisms for the function of 5-HT in learning and memory, which may be a useful target to benefit these patients with cognitive disorder.