Calcium signals in the nucleus accumbens: Activation of astrocytes by ATP and succinate
1 Department of Neurochemistry, Institute of Biomolecular Chemistry, Chemical Research Center, Hungarian Academy of Sciences, Pusztaszeriút 59-67, 1025 Budapest, Hungary
2 Neuromorphological and Neuroendocrine Research Laboratory, Semmelweis University and Hungarian Academy of Sciences, Tűzoltó utca 58, 1094 Budapest, Hungary
3 Department of Ophthalmology, University of Utah, 65 Mario Capecchi Drive, Salt Lake City, UT 84132, USA
4 Department of Zoology, Eszterházy Károly College, Leányka utca 6, 3300 Eger, Hungary
BMC Neuroscience 2011, 12:96 doi:10.1186/1471-2202-12-96Published: 3 October 2011
Ca2+ signalling evoked by ATP in the acute NAc slice from the rat brain. Movie of time measurement showing ATP (100 μM) application onto a Fluo-4 AM loaded acute NAc slice (300 μm). ATP was applied for 60 s (long puff) through a glass micropipette right above the tissue surface. Image acquisition frequency was 2 s in depth of 25 μm from the slice surface. Olympus FluoView300 software collected .tiff file stacks were coloured and converted to .avi file by ImageJ 1.44 (32-bit) image processing and analysis software . ATP-responsive cells were counted by using an ImageJ macro developed to average stacks before the time of ATP application and to subtract this average from every stack of the 10 minute-long recording. In this way, we got a 10-minute-long movie showing only the cells that had a fluorescence intensity change.
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