Additional file 3.

Stimulation of TG WT and TG G93A microglia with 1 mM ATP. (A) Intracellular calcium concentration ([Ca2+]i, μM) changes in NTG (WT) and TG WT microglia following extracellular stimulation with 1 mM ATP for 1 min. The period of ATP challenge is indicated with the bar. (B) Changes of [Ca2+]i in NTG (G93A) and TG G93A microglia following extracellular stimulation with 1 mM ATP for 1 min. The period of ATP challenge is indicated with the bar. (C) Area-under-the-curve (AUC) values (μM*s) for NTG (WT), TG WT, NTG (G93A) and TG G93A microglia. Data are mean ± SEM; Student's t test, *p = 0.0201 versus NTG (WT) microglia. (D) [Ca2+]i values (μM) recorded for NTG (WT), TG WT, NTG (G93A) and TG G93A microglia at 130 s of recording (10 s after initiation of 1 mM ATP challenge). Data are mean ± SEM; Student's t test, *p = 0.0381 versus NTG (WT) and **p = 0.0015 versus NTG (G93A) cells, n = three NTG (WT) mice (14 cells) and three TG WT mice (18 cells) from two litters, three NTG (G93A) mice (43 cells) and three TG G93A mice (38 cells) from two litters.

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Sargsyan et al. BMC Neuroscience 2011 12:91   doi:10.1186/1471-2202-12-91